How do you measure enzyme activity of immobilized enzyme on Fe3O4 magnetic nanoparticles?

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Baskar Thangaraj

I used APTES functionalized magnetic Fe3O4 nanoparticles as substrate and NS10086 novozyme immobilization. I removed the aliquotes completely by magnetic field and particles washed several times with PBS (pH-7.0) solution. I need the protocols or procedure and its formula for enzyme activity of immobilized enzyme.

I used tributyrin as a substrate and NS10086 enzyme.