For best solution characterization data is required. Hydrophobic, hydrophilic and lypophilicity value of the compounds lead to get a proper solvent. Lets try using ethanol/tween 20/tween 80 as 10 mg/ml of stock. 10 mg compound mix with 1 ml of solvent. I think you will get good solution.

try to dissolve it in the 2eq.HCL. 

 Solution: compounds are Hydrophobic, hydrophilic and lypophilicity value of solvent.

Prepare solution:

Ratio of  ethanol/ tween 20/tween 80 as 10 mg/ml to 20 mg/ml of stock. 10 mg compound mix with 1 ml of solvent.

    OR

Try to Dissolve or used different concentration of   HCl 

Read following points;

Electroporation is one of the most common methods used transform mammalian cells with plasmids. This method is versatile and can be adapted to meet the requirements of many cell lines. However, sometimes the efficiency of this method is low. We demonstrate that dimethyl sulfoxide (DMSO) facilitated a better DNA uptake in four different cell lines (HL60, TR146, Cos-7 and L132). The cells were electroporated with a beta-Gal expression plasmid in a medium containing DMSO (1.25%) during, and for 24 h after the pulse. In all these cells a dramatic (up to 8-fold) increase in transfection efficiency occurred after this treatment. This method opens up the possibility of using electroporation even in cells which are difficult to transfect.

Electroporation is one of the most common methods used transform mammalian cells with plasmids. This method is versatile and can be adapted to meet the requirements of many cell lines. However, sometimes the efficiency of this method is low. We demonstrate that dimethyl sulfoxide (DMSO) facilitated a better DNA uptake in four different cell lines (HL60, TR146, Cos-7 and L132). The cells were electroporated with a beta-Gal expression plasmid in a medium containing DMSO (1.25%) during, and for 24 h after the pulse. In all these cells a dramatic (up to 8-fold) increase in transfection efficiency occurred after this treatment. This method opens up the possibility of using electroporation even in cells which are difficult to transfect.

The objective of this study was to characterize Huh7 cells' baseline capacity to metabolize drugs and to investigate whether the drug metabolism was enhanced upon treatment with dimethyl sulfoxide (DMSO). 2. The messenger RNA (mRNA) levels of major Phase I and Phase II enzymes were determined by quantitative real-time-polymerase chain reaction (RT-PCR), and activities of major drug-metabolizing enzymes were examined using probe drugs by analysing relevant metabolite production rates. 3. The expression levels of drug-metabolizing enzymes in control Huh7 cells were generally very low, but DMSO treatment dramatically increased the mRNA levels of most drug-metabolizing enzymes as well as other liver-specific proteins. Importantly, functionality assays confirmed concomitant increases in drug-metabolizing enzyme activity. Additionally, treatment of the Huh7 cells with 3-methylcholanthrene induced cytochrome P450 (CYP) 1A1 expression. 4. The results indicate that DMSO treatment of Huh7 cells profoundly enhances their differentiation state, thus improving the usefulness of this common cell line as an in vitro hepatocyte model.

For cells seeded in scaffolds, transplanted cell survival rate plays an important role for cell transplantation efficiency, and is essential for successful cell transplantation. Fibroblast viability in HyStem-C was examined by a double staining Live/Dead Viability/Cytotoxicity assay, and cell images were analyzed using MetaMorph software for calculating live cell percentage for fresh and cryopreserved cells at different incubation time points, delivery methods, differing DMSO and cell concentrations. The results of this research demonstrated that in HyStem-C, the viability of cryopreserved cells (85%) was significantly lower than fresh collected cells (96.7%). In addition, the physical force from a 27 gauge needle significantly decreased frozen cell survival rates to 83-85% compared to pipette delivered cells. Higher DMSO concentration (1.0%) and higher cell density (2 × 107 per milliliter) also significantly decreased cell survival to 73%. Cryopreserved cell viability in three dimensional scaffolding can be maintained over 80% with cell density of 1 × 107 per milliliter, total DMSO concentration of 0.5%, and passed through a 27-gauge needle. These results demonstrate the viability of cells seeded in hyaluronan hydrogel with commonly used storage and delivery methods can bring rather satisfactory cell transplantation efficiency.

The specific conductivities and viscosities of lithium perchlorate at four different concentrations in dimethyl sulfoxide (DMSO)-based, binary mixed solvents are reported at 25 C. The co-solvents are tetrahydrofuran (THF), 1,2-dimethoxyethane (DME) and 1,3-dioxolane (DOL). The change in viscosity with solvent composition in all three mixed-solvent systems without electrolyte indicates the occurrence of structural disruption. The observed increase in viscosity with increase in LiClO4 concentration suggests an increasing compactness of the structure. A 2 M LiClO4 solution shows maximum conductivity at 20 vol.% DMSO in DMSO + DOL and at 40 vol.% DMSO in DMSO + THF mixtures. By contrast, a 1.5 M LiClO4 solution has maximum conductivity at 40 vol.% DMSO in DMSO + DME mixtures. Preferential solvation of LiClO4 by DMSO occurs in the respective mixtures with THF, DME and DOL.

Dimethyl sulfoxide (DMSO) is an important aprotic solvent that can solubilize a wide variety of otherwise poorly soluble polar and nonpolar molecules. This, coupled with its apparent low toxicity at concentrations 1% (v/v), using annexin V, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and AlamarBlue cell viability assays. DMSO concentrations >10% (v/v) have recently been reported to cause cellular toxicity through plasma membrane pore formation. Here, we show the mechanism by which low concentrations (2-4% DMSO) induce caspase-3 independent neuronal death that involves apoptosis-inducing factor (AIF) translocation from mitochondria to the nucleus and poly-(ADP-ribose)-polymerase (PARP) activation. These results highlight safety concerns of using low concentrations of DMSO as a solvent for in vivo administration and in biological assays. We recommend that methods other than DMSO are employed for solubilizing drugs but, where no alternative exists, researchers compute absolute DMSO final concentrations and include an untreated control group in addition to DMSO vehicle control to check for solvent toxicity.

EXAMPLE:

Ketoconazole is a weakly dibasic synthetic imidazole with a broad spectrum of antifungal activity. Topical, subconjuctival, and systemic administrations provide poor concentrations in the vitreous. To determine the ocular toxicity of intravitreal ketoconazole, the authors injected doses varying between 15 micrograms to 2240 micrograms dissolved in 0.1 ml of 100% DMSO into the vitreous bodies of 24 rabbits. Doses of 540 micrograms or less resulted in no toxicity to the eyes. Doses of 720 micrograms revealed electroretinographic abnormalities and photoreceptor degeneration by electron microscopy. Doses of 2,240 micrograms resulted in severe histopathologic and electroretinographic alterations. This study suggests that a single intravitreal dose of 540 micrograms or less of ketoconazole in DMSO may be safely used in the treatment of fungal endophthalmitis.

Dimethylsufoxide (DMSO) is a potent sensitizer of irradiated bacterial spores (Bacillus megaterium). It is effective under either anoxic or well-oxygenated conditions; in both cases, DMSO increases the response by a factor of 3 to 4. In water, the oxygen enhancement ratio (OER) with 260-kVp X rays is 1.9, while in pure DMSO, which is not toxic to spores, the OER is reduced to 1.1. Spores exposed to DMSO and then washed and resuspended in water still show most of the DMSO-characteristic sensitization, even when the spores are soaked in water for 24 hr before irradiation. This effect is not attributable to DMSO retention by the spore; instead, we suggest that DMSO causes a long-lasting change in a critical spore component that changes the way the entire cell responds to radiation.

Hello Mayank,

I didn't use BIBN 4096 but I came across this kind of solubility issues and I solved them.

Solution I #

1. You have to lyophilize  your sample (for recovery)

2.  Try to dissolve your sample in pure DMSO sonicate if you need

3. Dilute with water now.

Solution-II #

Your compound looks hydrophobic and it contains primary and secondary amines so make either HCl or Oxalic acid salt by treating with respected acids. Hopefully salts will dissolve in water (not always so) and make sure when you calculate  M.Wt of the compound include counter ion M.Wt as well.

Good luck