Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

Which variables can be used to increase/decrease the grain size of czochralski-grown polycrystalline silicon?

Which variables (such as pull speed, melt temperature, annealing temperature/duration, cooling rate, etc.) affect the grain size of czochralski-grown polycrystalline silicon? In what direction...

10 January 2021 2,204 6 View

How do I justify the use of fish scales to synthesize a nano filter?

Good day. I am doing a studu on the use of fish scales to create a nano filter. This is aimed at potentially solving the face mask problem brought about the pandemic. This is what I know so...

09 January 2021 8,907 5 View

Calculating Intercoder Reliability for 3 coders with 7 categories across 6 texts?

How would you calculate inter-coder reliability (Kappa coefficient?) for the following scenario: 6 texts coded across 7 categories (codes) by 3 coders?

23 December 2020 2,324 3 View

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10 October 2020 3,407 2 View

Do you agree that one of the advantages of the Boundary Element Method is that it easily addresses Incompressible Materials (Poisson's Ratio = 0.50)?

When modeling incompressible materials via the Finite Element Method (FEM), typically the plane formulation that is displacement based fails when Poission's Ratio = 0.50, which is a standard...

08 October 2020 7,058 1 View

Recommended analysis for an ANCOVA-like problem with binary variables for dependent variable and covariate?

What is your recommended analysis for the following problem: I have 3 (treatment) groups and a non-randomized pre-post design. I want analyze the effects of the groups on the follow-up measure,...

28 September 2020 5,890 4 View

No title

18 August 2020 3,955 11 View

Are there any test methods for butterflies or other arthropod pollinator besides bees?

In the current EFSA Guidance on the risk assessment of plant protection products on bees (Apis mellifera, Bombus spp. and solitary bees) related to the Regulation (EC) 1107/2009 valid test methods...

26 March 2020 1,178 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View