I intend to test the drug on mice models of diabetes and the drug i intend to use is an ayurvedic medicine and hence the active component is unknown to me. i do plan to do a chromatographic analysis to separate out its components. but am confused as to what path to follow so as to determine its mode of action.
You can measure levels of blood glucose and insulin before and after treating with your drug. If its hypoglycemic, blood glucose will show a decrease and insulin will increase in a certain time period. Once you get this result..you can pursue mode of action in different ways.
Yes, the amino glycosides are dastardly for wiping whole sectors of the hair cells in the basal (HF) region. I am profoundly deaf as a result of streptomycin and dehydrostreptomycin administration for rhuematic fever many years ago. But we did a pilot study (never completed the study itself) back in 2005 on NSAIs, Aleve (Naproxyn) in particular, and found an average shift in thresholds of 10dB per annum among daily users of them. It required very high doses of the Ibuprofen but just daily use of Naproxyn to make that shift. The VA ought to be looking closely at that as they give out NSAIs like candy.
1. Utilize OLD RATS.
2. USE THE DRUG AFTER A GLUCOSE LOAD.
That woud be the first Step.
Best Regards,
JM
And I think we will find the old rats dying an early death, just as the humans who take such metabolically interrupting chemicals will die earlier albeit over years rather than weeks while taking it. On a more serious note, a truly worthy scientific experiment of tremendous potential for good is going for enterically released organic substance or extract such as ginger or bitter melon, etc. Most of the drugs we have investigated with were synthetic versions of something natural. The synthetic versions were loaded with terrible side effects, and the natural versions rarely exhibiting anything of a serious nature. Hypoglycemic inducing compounds or substances, like those administering them, should first do no harm, in my opinion.