I am trying to create zebrafish lines with specific mutations using CRISPR-Cas9 and homologous repair templates. I have had a lot of success with CRISPR-Cas9 cutting right next to the loci of interest. My HRM results are giving me a melting temperature about 1 degree lower than wild-type which would correlate with my repair template having C>A and G>A mutations. I then sequence those positive HRM samples only to have the sequence come back wild-type. I see a lot of publications that do not seem to do any sequencing of their HDR organisms. Is there something I am missing in this method?