Kendal Prill

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Questions related from Kendal Prill

In situ hybridization - sense probes stained even though I had tissue specific staining with minimal to no background in antisense probes?

I had tissue specific staining in some of my embryos where stained structures are evident, such as the somite boundary in the picture included. I had other probes such as heart specific antisense...

10 January 2022 9,749 1 View

How do you confirm HRM results for SNPs generated via CRISPRs and Homologous repair?

I am trying to create zebrafish lines with specific mutations using CRISPR-Cas9 and homologous repair templates. I have had a lot of success with CRISPR-Cas9 cutting right next to the loci of...

08 August 2019 2,641 0 View

Has anyone else got HRM working on a Corbett Rotor Gene 6000 with Evagreen?

I am trying to get HRM working on a corbett Rotor Gene 6000 using Qiagen Type-It Kit. For some unknown reason, I cannot get this machine to detect any amplification and subsequently there is...

08 April 2019 5,791 1 View

What Preprint Server do you use or recommend?

I am trying to find a preprint server for a review I have written. I have been looking at Open Science Framework (OSF) but I cannot find any user feedback from authors who liked using it or the...

01 January 1970 4,965 3 View