Or you can take a reference sequence and pick up primers with one of the on-line packages - they are many, just google request.

What characterization do you want to do? We have a lot of probes to distinguish Leishmania species.

Thanks Rachel Schwartz. I have unknown Leishmania cultures isolated from skin lesions of patients clinically diagnosed with Leishmania, I am looking for specific primers to diagnose these isolates, whether it is Leishmania tropica or L major; also the same story for L. donovani specific primers. Any idea please? 

I bet we can do this. I have a collaborator who is working on this for ancient dna. She has the data for the actual probes she is using. Send me an email directly and I'll send you her contact information.

Hi

The are known generic primers (LISTR and L5.8S) to amplify the ITS1 gene of Leishmania species . Once amplified, you need either to sequence the gene or perform RFLP (using Hae III enzyme). If you have only one specimen, it will be easier to identify by sequencing.  You can find the sequence of these primers in the attached paper.Let me know if I can help.

Nabil 

Dear Hayder I agree with Nabil Haddad

if you have unknown Leishmania cultures isolated from skin lesions, you can use a RFLP-PCR to figure out the molecular characterizations of your unknown Leishmania isolates. I think It is doable using 2-way RFLP-PCR as mentioned in the following reference:

El-Beshbishy, Hesham A., Khalil H. Al-Ali, and Ayman A. El-Badry. "Molecular characterization of cutaneous leishmaniasis in Al-Madinah Al-Munawarah province, western Saudi Arabia." International Journal of Infectious Diseases17.5 (2013): e334-e338.

Hi Artem, Rachel, Nabel and Harith, 

Thanks all for your helpful answers, Rachel was so helpful and gave a very solid information via email. Harith, I don't think I am going to do RFLP-PCR as it is not easy at present but I have managed to find species-specific primers of target. Finger crossed for all.

Best wishes for all