We have developed a qualitative isotyping ELISA for mouse monoclonal antibodies. For QC purposes we need to decide appropriate cut-off values for a positive and negative result, what is the best way to go about doing this?
hope this is useful
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4981115/
regards
maximum 1.8 OD is recommendable. optimize the dilution of antibody of negative serum by checker board analysis. Negative sample should confirm by any ELISA kit. If you dont have any specific kit take the OD of positive(known like hyper immune or known by other method) and negative serum (suspected) at same dilution against same antigen. Many sample observe at same dilution or variable dilution according to need. then you can able to ..
Above the mean of your negative control sample by 3 stander deviations consider positive.
- Badges
- Science method
- Similar topics
- Medicine
- Immunology
- Immunology Techniques
- Immunoassay
- ELISA