I need a positive control for a cell cycle analysis experiment. Can anyone please suggest how you would arrest some cells in G0 to be used as a control? Thank you!
Dear Vishaka Gorur
you can synchronise cells at M phase using Nocodazole, colchicine, colcemid. if you want the cells to exit cell cycle and remain at G0 then you can try nutrient starvation.
this post might give you some other ideas as well- https://bitesizebio.com/33861/perform-cell-synchronization-specific-cell-cycle-phases/
good luck !
Dear Shambhabi Chatterjee,
Thank you very much for the very helpful suggestion and the link to the post!
Kind Regards
Vishaka
It depends on the cell line but most gave a good G1 block by 24h serum starvation followed by 18h (overnight) treatment with 100microM Hydroxyurea. Wash and replace the medium with full serum to release the block if required, and if you have a number of dishes or flasks then you can use flow cytometry to watch the populations travel through S phase in synchrony.
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