I was just thinking could it be possible just because 2- 5 hr storage of my yeast cells before soptting could switch to anaerobic or post diauxic shift
Sorry but your question is a bit confusing. The title of your question is asking about plates, but the text of your question seems to imply storing the cultures before plating on plates.
But there is adequate aeration on agar plate surfaces, at least until the colonies get large (there is oxygen deficient in the center of large colonies). If you are storing a culture in a tube prior to spotting then there is of course no aeration. Whether or not they switch to anaerobic growth depends on what they are stored in. If in neutral buffer then not much changes. If in medium then they might switch metabolic states. However this is not really of concern because you are then plating them on on plates in aerobic conditions so by a few generations they will have converted to aerobic metabolism.
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