10 Questions 4 Answers 0 Followers
Questions related from Shirish Kumar Gangber
I am happily using homologous recombination for candida genetic experiments then suddenly a question came in my mind why some people use crisper and other methods for gentic methods and major...
16 November 2023 2,892 0 View
why this multiple bands
21 October 2023 9,231 4 View
Why I am getting multiple bands please help if anyone have any idea of this
21 October 2023 5,172 6 View
In case of plasmid transformation gene vector could be empty for false colony but how in case of gene disruption based on homologous recombination can give false colony either antibiotic cassete...
18 October 2023 9,472 0 View
I was just thinking could it be possible just because 2- 5 hr storage of my yeast cells before soptting could switch to anaerobic or post diauxic shift
17 October 2023 4,935 1 View
I have disrupted yeast gene in genome now I want to verify it without delaying it for overnight culture and then genome isolation so can I directly check it (gene disruption )from transformed...
07 October 2023 1,313 3 View
I have disrupted gene in E colli genome can check correct disruption in genome of e coli by colony pcr I had yet done colony pcr for plasmid gene amllification Or verification what about genome...
07 October 2023 3,536 2 View
I was done the 2hr restriction digestion of a plasmid but in gel run no fall out came instead a accumulation of something observed at bottom as in image what could be the possible reason
30 September 2023 7,079 2 View
I had extracted dna from E. Colli which shown very low digestion even with Hf digestive enzyme It is suspected that becouse of 1-3 minute kept plasmid with Pd3 during plasmid Isolation caused...
14 September 2023 6,871 3 View
I had very few amount of my target Gene purified by gel extraction now to amplify gene I did pcr of the same.... so can I use this Pcr product directly use to my restriction digestion reaction.....
07 September 2023 9,552 1 View
