Hi- I am a PhD candidate at the University of Florida and have run into this issue many times. Essentially, the Qubit is a more accurate reading for determine the conc. of DNA in a sample versus the Nano because Nano detects TOTAL nucleic acids (everything in your sample), whereas the qubit dyes bind directly to the target that your are measuring. In other words, if you are measuring double stranded gDNA for PCR amplification and you are using the dsDNA HS kit, that is exactly what you will be measuring. I hope that is helpful.

If you have any more questions, please feel free to message me.

Cheers and goodluck!

Ks

https://www.researchgate.net/post/Qubit_vs_Nanodrop2 Discussed here.

We are doing quantitation of DNA by using Qubit as it is more specific that nano , as katherine mentioned .. but you need to calculate the results accurately by changing the volume and the units at the final screen ..

Hope this could help

Thanks you all for your information

Yes, I think for DNA concentrations of our samples the Qubit is preferable.

But, we will compare the two methods in a large sample screening to find out the correlation between Qubit and nano drop.

I will keep you informed

thanks again

I agree with Katherine.

We use the nano drop, but in my experience it is not so accurate.

Sometimes the measures with the nano drop are variable if the DNA samples are still cold. We measure the same sample several times to obtain an average of the DNA concentration.

Hope this help,

Best regards.

Dessireé, thank you

We are using picodrop but its not so accurate. I think that anyother spectophotometry method is better than picodrop. Good luck!

there 2 more Technics to know ur DNA con

one is run ur DNA in agarose gel

second take spectiphotometric reading other than nano drop