How do we get confirmed that the isolated RNA from a fresh leaf sample is of good quality?
There are several complementary ways to do this:
- Run an agarose gel, check for the integrity of the RNA ribosomal bands (there should be no smear).
- Measure the absorbance in a Nanodrop or similar spectrophotometer, look for the standard values for the indexes.
- Qubit fluorometer is also used for these purposes(very helpful for us).
- If sequencing of the nucleic acids is intended, an Agilent 2100 Bioanalyzer is the best choice.
Respected sir, can you suggest me a research journal to communicate the research article on the topic entitled “High-quality RNA isolation protocol in FOC race-4 infected Musa paradisiaca cv. Malnad Rasbale for NGS analysis “.
Article The RIN: an RNA integrity number for assigning integrity val...
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Biology
- Biomolecules
- Nucleic Acids
- RNA