How do the capture antibodies attach to surface of polystyrene plate well in ELISA?

24 August 2016 1 6K Report

What are the specific interaction involved in coating the capture antibodies on a plate well?

I see it is a 4 year late reply. But for others who are looking for same info and land on this page like me, this might help:

https://www.corning.com/catalog/cls/documents/application-notes/CLS-DD-AN-454.pdf

Badges
Science topic

More Aditya Kurdekar's questions See All

I am trying to obtain microstructure for Mg-Zn-Sn alloy?

Any suggestions with respect to etchant composition and holding time?

27 July 2024 6,925 2 View

In bismuth borate glasses concentration of boric anhydride is changed with some other, WHY?

In Bismuth borate glasses, concentration of boric anhydride is changed always, instead Li2O of ZnO or CdO. Why ? In most of research journals concentration of network formers are changed metal...

10 June 2024 4,234 0 View

What amount is good for making an electrodes in supercapacitors? Many of the authors used 0.5 mg and other used more than that why?

Is there any advantage/disadvantage for choosing less amount (i.e., 0.5 mg) Or in choosing higher amount (1 or 2 mg) ?

06 June 2024 6,938 2 View

How to colour code depth information in image J software?

i have tried processing the z stack confocal image to represent the various depths of the sample by using Depth coding (Image › Hyperstacks › Temporal-Color Code). But the scale bar i get is in...

23 May 2024 9,618 0 View

What are the most effective techniques for mitigating overfitting in neural networks, especially when dealing with limited training data?

11 April 2024 8,176 2 View

Is it possible to make predictions utilizing Artificial Neural Networks (ANN) for a solitary univariate variable using python?

Forecasting using ANN for a single variable. Say, inflation.

03 April 2024 6,530 4 View

How to improve the adjusted R square value of a multinomial logit model (MNL)?

I am performing multinomial logit (MNL) on discrete choice data. I wanted to understand how I could improve the value of the R square of my model.

30 March 2024 9,304 2 View

What role does the complexity of the dataset play in the susceptibility of deep learning models to adversarial perturbations?

09 March 2024 713 1 View

What approaches can be used to enhance the interpretability of deep neural networks for better understanding of their decision-making process ?

What approaches can be used to enhance the interpretability of deep neural networks for better understanding of their decision-making process ? #machinelearning #network #Supervisedlearning

04 March 2024 8,100 3 View

What are the challenges and opportunities in deploying machine learning models in real-time systems with stringent latency constraints?

What are the challenges and opportunities in deploying machine learning models in real-time systems with stringent latency constraints? #ml #industry5.0

04 March 2024 1,688 1 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View