How to compare different models with different inputs and data length?

I want to run 5 different models to estimate stream flow. In order to optimize the characteristics of these models I use Taguchi method. So I have to run different models according to the Taguchi...

11 February 2021 7,986 6 View

How to find strain energy release rate GI, GII, GIII in Abaqus?

I modeled a simple crack in abaqus. I get J-integral as output as well as components of stress intensity factor KI, KII, KIII. However, I do not know how to get GI, GII and GIII. I searched in...

04 January 2021 1,783 2 View

What is the best way of moscaicing overlapping images?

I have several images with overlaping areas. I try to mosaic them using rasterio.merge library. But rasterio.merge funtion replaces those overlapping pixels with "nan" values. I then tried both...

29 December 2020 9,849 1 View

How can I check the correlation between a group of features and one feature?

Hello all, I have a dataset that contains some pressure meters in a water distribution network and all valves of the network. Since I do not have the graph of the water distribution network, I...

30 October 2020 2,333 4 View

Any idea why my Western Blot Stripping buffer doesn't work?

I activated my membrane twice in total and hybridized it with two different antibodies. The first antibody was the specific antibody I was looking for and the second one was GAPDH. I then...

29 October 2020 5,530 6 View

Changing TEM mode to TE and TM modes?

Hi there, I would be grateful if someone could let me his opinion about this question: There is a coplanar stripline waveguide excited with a TEM mode. My question is that how to create TE and...

11 October 2020 5,816 3 View

I have problem with my SDS gels in western blot and wondering anyone could help?

So when I prepare my 10% SDS gel for Western blot, I see that my resolving solution is perfect but my stacking solution does not seem to be working properly. So when I take the comb out, the wells...

17 September 2020 3,355 7 View

How can we optimize a water distribution system if we do not have the zones' topology (pipes graph )?

Hello everyone, My issue is about a water distribution system that I am working on a zone of the system where does not exist any plan for the place of pipes. However, we have the place of...

14 September 2020 5,978 6 View

Whats this plant?

Dear All Hi I wanted to know the scientific name of this plant species. would you please help me to identify it. Actually, I found it in the area in the East of Iran.

21 August 2020 6,705 2 View

Which version of Mplus is suitable to assess the exploratory factor analysis of a questionnaire with 20 items?

Dear Researchers and statistical analysts, Which version of Mplus is suitable to assess the exploratory factor analysis (EFA) of a questionnaire with 20 items? I'm working on initial validation of...

14 August 2020 3,341 4 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View