Problem in detection of a specific gene & elimination of primer dimer in qpcr?

In our lab, we are trying to do real time PCR for some genes. One of them has a size of 85 bp. Whatever we do, we had an amplification in negative control. When we run agarose gel, it always a...

14 February 2021 9,750 11 View

How can critical thinking be developed in scientific research?

Dear researchers, As I know, critical thinking is an essential feature that should be in scientist. Can you tell me how this skill can be developed? or how do you gain it from your long research...

16 September 2020 2,608 4 View

Online miRNA target prediction tool and related- down stream pathways?

There are many miRNA target prediction softwares, can you help me from your experience which one is the best? What are the consideratios I must take? because I have no experience in it...

02 September 2020 6,714 5 View

Fault analysis with Simulink- abnormal response after fault clearance?

The attached simple model with SIMULINK after clearing the fault, the Simulink is having abnormal responses. Bus voltage V3 is oscillating. anyone facing that issue?

09 April 2020 2,882 3 View

Continuous IV, DV, M and categorical control variable in mediation. How do you proceed?

Hi everyone! I ask you how you would proceed in the case in which, for a mediation analysis, all considered variables (X, M, Y) are continuous while a control variable to test is categorical (not...

15 January 2019 5,073 3 View

Can I appeal against a journal editorial decision?

Can I appeal against a journal editorial decision?

28 November 2018 8,076 50 View

How to convert Ti (integral time) and Td (derivative time) to I and D parameter in function block parameter for PID controller (SIMULINK)?

I need help on my problem. I managed to tune my PID controller using Ziegler-Nichols continuous cycling method and able to determine my Kp, Ti (integral time) and Td (derivative time). But the...

22 May 2017 5,641 8 View

What is the difference between generalised additive model and polynomial regression?

13 June 2016 6,758 5 View

How can I put cluster labels in the Gaussian Mixture Model (GMM)?

I used GMM to cluster my data. It has 4 dimensions with 3 clusters. The GMM ran very well, and I got the mean and covariance matrix for each cluster. The problem is I don't know which label...

15 October 2014 7,213 5 View

Where can I find researchers interested in education reform and health problems affecting students caused by problems in education?

I'm extremely interested in issues concerning students' heath in educational settings such as stress levels in class rooms and how standardized testing affects drug use on campuses. I would like...

27 July 2013 7,421 3 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

Is it Ok to decline to write a chapter for a IntechOpen to the lack of experience?

I have been asked by IntechOpen to write the chapter for a book they are going to publish, but as I don't have enough experience in the area they proposed me to write I declined their offer. Now...

24 February 2021 2,802 4 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

What is the minimum number of studies in each category required for a multiple subgroup analysis to be viable in a meta-analysis?

Cochrane Handbook mentions subgroup analyses are only warranted if there are more than 10 studies (I have 28), and if they are distributed evenly among categories of subgroup. I am wondering if...

17 February 2021 3,467 4 View

Is it appropriate to use AIDA model (Assessment of Identity Development in Adolescence) on a non-clinical sample?

I am researching emotion regulation and identity development in adolescence. I've come across several inventories for assessing identity development and it seems like the AIDA scale is the most...

16 February 2021 1,261 1 View

Defining mixed model with repeated measures data?

HI all, I have been working with daily diaries data ( 6 emotions of which 3 positive and 3 negative) were assessed for 30 days. These observations (1level data) are then nested within subjects...

13 February 2021 1,810 3 View

Do you know of an informal caregiver of a person who has recently received a bone marrow transplant?

We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...

13 February 2021 9,174 1 View

I'm doing research concerning teacher burnout and COVID-19. Can you please answer my questions (see description)?

Dear friends! I am holding a research on teacher burnout during the COVID-19 pandemic. Please answer my questions: 1. Country you come from 2. Years of working as a teacher 3. Have you experienced...

07 February 2021 8,325 10 View

Am I doing right for my literature review?

Hi, I am working on my literature review for my thesis. There are very limited numbers of paper in this field. Therefore I feel like I am pulling information with a tweezer. I am working on the...

04 February 2021 4,277 3 View