How do I stop TEM samples being destroyed under electron beam?

20 August 2019 1 2K Report

The samples that I make for TEM contain cells. I need them to be 70-90nm (no higher) to be able to see them clearly and do EDX as well. However, They begin to rip and flex under the beam even at 6000x. I have carbon coated before adding the samples to the copper grids and then afterward as well.

How do I increase protection of my samples so I can go to higher magnifications?

increase coating thickness? (mine is 2nm currently)

buy coated grids instead?

Göran Stille

Have you tried using lower electron beam current? Thus exposing your samples to less energy input per second. Will require longer times of exposure.

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