FLICA assay not working?

Hi everyone, I am trying to perform the FLICA apoptosis assay with my MSCs. However, although the cells look healthy upon inspection and the viability is greater than 90% with cell counting, the...

17 January 2021 6,959 1 View

Can anyone help me in doing whole mount immunostaining on mouse colon?

I am exactly looking forward to know the procedure to isolate the myenteric and submucosal layer from intestinal whole mount. I would like to see the degeneration of dopaminergic neurons on it?...

12 October 2020 6,069 2 View

Can a RFU plot have no Ct value in a qPCR reaction?

Hi, We have a small test run for a set of RNA samples with 3 fluorophores, FAM, HEX, and Cy5. FAM and HEX are for test samples while Cy5 is with internal control. Surprisingly, for some of our...

03 September 2020 7,713 3 View

Poisson's ratio of nano hydroxyapatite?

I have powder of nano-hydroxyapatite with average particle size of 100 nm. I want to find the poisson's ratio of nano-hydroxyapatite.

30 June 2020 7,704 5 View

What are the factors affecting Wind Energy Production in Denmark?

Hi everyone, I am a Master's student and I am doing my thesis on Wind Energy Production forecast for Denmark and for that I need the data of the Factors affecting the Wind Energy Production in...

06 November 2019 7,535 2 View

Pls suggest topic for mtech final yr project in quality management as i m working in conveyor belt industry?

..........................................................

06 November 2019 4,614 3 View

Sericin enzyme is typsin or cysteine?

biotechnology enzymes

30 March 2019 8,612 3 View

How to fix rigid body in ansys workbench ?

I am evaluating deformation behavior of porous block of 10*10*10. For this i put this model between 2 plates. Stiffness behavior for plates I had given is rigid. Form the target node from top...

18 September 2018 4,594 4 View

How to view the solution model for parameterization of design in ANSYS?

I am doing parametric analysis. Well i got the solution of all three parametrized values. How I can visualize for all the solutions. Visualization of model analysis is showing only first...

10 September 2018 3,489 4 View

Cross signal overlap calculation?

I want to calculate cross signal overlap between two signal located at +f and ant -f. image of one signal distort the other signal. so how to calculate this distortion.

29 March 2018 9,174 2 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View