I am treating cultured AVCMs from mice and analyzing gene expression with a collaborator. Normally, I would just isolate the total RNA from the culture wells and perform PCR. In this specific case, I need to ship either the cardiomyoctyes or the extracted RNA to a collaborator. If I extract the RNA then I need to freeze and ship frozen samples. Less than ideal.
My other option is to put the cardiomyoctyes in RNAlater. If we use RNAlater, would you scrape the cardiomyoctyes in RNAlater or trypsinize, pellet and resuspend them in RNAlater? I have never had a need to trypsinize primary cardiomyoctyes before and scraping primary cardiomyoctyes seems a bit aggressive. If I recall we had a bit of an issue getting a cell pellet (HL-1 cells) out of RNAlater in the past.
Any suggestions are appreciated...
Hi Chad - Unless courier costs are an issue, I believe that isolating the RNA yourself and then shipping it on dry ice would be best for maintaining integrity. I've had no problems with this method. I can't comment on sending samples in RNA later.
Best of luck
hi dr Chad,
the ideal way is instead of isolating the RNA u cud rather directly ship or courier the cardiomyocytes to ur collaborator
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