I have four diastereomeric pairs to separate on chiral HPLC, out of which one diastereomer is of interest. One pair already separates on IB column, but two enantiomers need to be separated from the other three diastereomers. The compounds have a peptide bond and no functional groups.
What should my strategy be and which column is suitable for peptides?
It is unlikely that all four pairs would separate out using one method on one column. As I have done this many times in the past, there is one primary strategy to use if you are only interested in ONE diastereomer. Use an achiral C18 style column to separate and collect the one pair of interest (or if you need them for other work, all four diastereomeric pairs). Then develop a chiral method for the pair of interest.
Thanks for your suggestion William !
As we were looking for Tech-transferring this method, we were trying to resolve it in minimum no. of columns..But as you said it is really tough.We even tried some gradient methods..but didn't worked well
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