How to prepare a 0.2 M phosphate buffer?

I need to prepare a 0.2 M phosphate buffer. In my research, I've seen people prepare it with both monobasic and dibasic sodium phosphate... is this necessary? Can I just use one or the other?

13 September 2020 4,656 2 View

Non-parametric equivalent of three-way ANOVA for small sample size?

I inherited a dataset with a very small sample size (n=3 per group) and three factors (primary insult x secondary insult x treatment). I intended to use a three-way ANOVA, but I was warned that...

31 March 2020 5,913 3 View

How do I obtain a doi for my publication?

A researcher here at my institution is asking about getting a doi for his publication to increase its findability and exposure. Does one have to go through the avenue of a Registration Agency? If...

04 February 2020 8,760 6 View

Does genomic DNA bands smear on gel are degraded DNA?

I have to send my sample for metagenomics sequencing but my genomic DNA shows up a clear band with thin and long smear. It looks like my sample has degraded but I'm not sure. I read somewhere that...

15 December 2019 206 3 View

How to summarize reliability values ?

Hello, I am coding some metrics from different articles to run a meta-analysis and I had a simple question. Let's say one of my variable of interest is Brand loyalty. In some articles, brand...

19 August 2019 171 2 View

Ways to dry phenolic extracts in 70% acetone (2mL samples in 96 deep well plates)?

We are extracting phenolics using 70% acetone. The samples are set up in 96 deep well plates. The CentriVap (rotary vacuum) and lyophilizer that we have are made with materials that could be...

02 September 2018 3,880 3 View

Was the heavy cavalry of Alexander the Great the first cavalry to charge home in combat against unbroken enemy cavalry?

18 August 2018 7,200 6 View

Methods for sterilizing (killing biota on) granular activated carbon (GAC) ?

I am only interested in methods that don't change the GAC's surface chemistry or absorption capacity. The ionising and non-ionising irradiation methods we have looked into seem likely to affect...

03 April 2018 5,313 3 View

Probability of viral plaques to skip a dilution at low levels?

Hello all, I'm looking to calculate the probability to have viral plaques appear in lower dilutions but not in the previous dilution. We perform serial 10 fold dilutions (0.2 ml into 1.8ml...

20 February 2018 2,350 2 View

Recommendations needed: 3D printing for research?

- Does anyone have experiences in geosciences regarding sample printing? What type of printer are you using? What type of material could be interesting. We are thinking of PVA and PLA combination....

18 October 2017 3,876 3 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

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01 March 2021 3,905 5 View

Hello house, is it necessary to compute Content Validity Ratio (CVR) for an adapted questionnaire pls?

What should one do if he obtains conflicting results between Content Validity Index (CVI) and CVR for items in a quetionnare. For example based on the CVI obtained the items are acceptable but the...

25 February 2021 7,325 2 View

Is there any teachers based in UK who would like to take part in my study?

24 February 2021 4,833 3 View

Anyone give me assistance with analysing Gait Data?

I currently have collected Gyroscope/Accelerometer data (X/Y/Z) for my dissertation, but not very knowledgeable on how to process my data and apply Machine Learning? Can anybody help?!

24 February 2021 9,610 3 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Inpatient DBT to reduce parasuicidal behaviour?

Currently trying to obtain as many pieces of research as possible for my dissertation around the implementation of DBT within the inpatient unit for those with a diagnosis of BPD. Primarily...

23 February 2021 5,654 2 View

What type of SPSS analyses should I use for my Dissertation?

I'm currently working on my Dissertation and I'm trying to think of the best analyses that I should use for my study. I'm using three questionnaires for this and two different groups, 1st group...

22 February 2021 1,351 4 View

Is it correct to identify an air mouvement by the Venturi effect in buildings ?

Knowing that the Venturi effect is used most in tubes (according to my basic knowledge in physics) to increase the speed of air or liquids by reducing the tube volume, my question is if we have...

17 February 2021 1,260 1 View