How do I screen peptide ligands from bacterial display library which binds to a mammalian membrane protein (receptor, not an ion channel or GPCR)?

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Farkas Kerényi

Look at this:

http://www.sciclips.com/sciclips/bio-protocols.do?catName=Protein%20display/Protein%20engineering/Directed%20evolution&l2CatName=Bacterial%20surface%20display

And this:

http://www.chemie.tu-darmstadt.de/kolmar/science_1/bacterialdisplay/bacterialdisplay_1.de.jsp

Mitan Sutradhar

Thanks Farkas for your answer, but I had already considered this option, as in successive rounds of MACS and FACS but the issue here for this approach is that the receptor needs to be in soluble form attached to a bead, which is not possible in my case as the receptor is an integral membrane protein and hence cannot be purified in its original conformation. is here any way that i can study the interaction of ligands with the receptor in its original conformation??

His/Flag/Myc/etc. tag on receptor?

yea..that seems fine...i was wondering if I could use SPR for this.any suggestions?