Mitan Sutradhar

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Questions related from Mitan Sutradhar

Are there any explanations how my electrocomtetent TOP10 cells have become both ampicillin and kanamycin resistant?

The colonies for innoculation were tested for resistance and they did not grow on amp and kan LB agar plates, but magically somehow the electrocompetent cells made from them are resistant to both...

07 July 2013 5,579 5 View

How do I screen peptide ligands from bacterial display library which binds to a mammalian membrane protein (receptor, not an ion channel or GPCR)?

The peptide display library employs NNS mutagenesis, if that is of any help. What techniques can be used to show interaction and hence screen peptide ligands?

06 June 2013 1,013 5 View