QPCR standard curve troubleshooting advice?

Hi all, I am trying to set up my standard curve for qPCR analysis, presence absence of D. septosporum, using a QuantStudio 5 real-time PCR system and associated software. I am using a 5X...

25 November 2020 710 3 View

Can anyone help with setting up the R germinationmetrics Package using multiple species and treatments?

I have 14 species, 6 temperature settings and two treatments to examine germination responsiveness over 59 days. I want to start with some basic info for plotting (t50, Gmax etc). I want to...

18 November 2020 7,160 4 View

How to extract patient specific 3D geometry from GE Vivid E95 3D/4D echo?

I am needing to obtain patient specific geometries of the left ventricle for use in CFD and 3D printing. Does anyone know how to do this using 3D/4D echo obtained from the GE Vivid E95? Does this...

29 September 2020 2,614 3 View

Ideas for knocking down protein that is heavily upregulated in cancer?

Hello all, I am trying to knock down a protein that has been documented to be heavily upregulated in oral squamous cell carcinoma (confirmed via western blot). I have attempted to knock down this...

31 August 2020 9,214 3 View

How to calculate error in photocatalytic data?

Hello, I made some measurements using a UV-vis spectrometer at intervals of 5 minutes to see the degradation of a resazurin dye by TiO2 under UV light. I did not repeat these measurements. How can...

23 May 2020 1,373 3 View

What is the ideal retention interval for measuring retention of knowledge?

If you're using an intervention in classrooms and testing knowledge at pre-intervention, post-intervention (immediately after) and then again at a retention time period, how long is the ideal...

19 May 2020 307 8 View

What is the best way to quantify the amount of a bispecific t cell engager (BiTE) present?

I'm currently working on a project to study BiTEs, but I'm struggling with the best way to quantify the amount of BiTE being produced by my cells. Any help would be greatly appreciated. I've...

23 February 2020 9,189 1 View

What statistic do I use on non-parametric data where I have a response variable and two interacting categorical explanatory variables?

I was wondering whether anyone had any suggestions as to what statistic to use (I use R Studio). I have some muscle cell circumference measurements (response) and I have two explanatory variable...

27 January 2020 5,835 3 View

Can someone please explain the credibility behind Travelodge's survey: "The Secret to a Good Night's Slumber is to Sleep in a Blue Bedroom" ?

Currently, I'm investigating the effect bedroom color has on one's quality of sleep. Many (non scholarly) works I've come across have mentioned / cited this survey, conducted by Travelodge in...

21 September 2019 4,088 2 View

Is there a way to simultaneously visualize DNA and protein following electrophoresis?

I am currently optimizing the conjugation of a DNA oligo sequence to a DBCO activated antibody and am looking for a method which could allow me to simultaneously visualize the amount of DNA which...

15 May 2019 9,228 3 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

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03 March 2021 5,360 2 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View