Is there any specific standard method to quantify 70% ethanol soluble protein extracted from cereal flour?
If the amount of protein in your flour is low, i think you might need to use a sensitive method that can detect such amount. E.g., Bradford Method with BSA for standard.
Bradford test is usually done with samples dissolved in water right? Can it be done for protein dissolved in 70% ethanol as well??
Dear Subin,
I think that It is better to try the Lowry Method, It is very sensitive method in determination protein content, your sample in 70% EtOH, so you must prepare your blank solution with 1 ml 70% EtOH, and also you must dilute your sample with 70% EtOH again, for instance you can prepare dilutions like 0.1 ml + 0.9 ml 70%EtOH or 0.25 ml + 0.75 ml 70% EtOH. 70% EtOH will probably not cause any interruption while measuring in spectrophotometer. In addition, you must also use 70% EtOH while preparing the standard graph with the standard prolamin protein. I hope that I could help you.
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