Hi all,
I would need to compare the number of cells and fibers in different part of a tissue which was used in fluorescent immunohistochemistry. However, I am not sure how to proceed. I could use image J to count the number of cells but I am more doubtful to count the number of fibers. Does someone have an idea? Thank you very much for your hep !
Image J should help. You should try harder to find a way to quantify your fibers using Image J
Image J should work (just find the suitable plugin) but it's better to state specifically what kind of fibre it is for us to help a bit more.
Do you need to know the number of fibres or would it be useful to measure the area covered by fibres instead? You could do this by setting a threshold for the fibre fluorescence then using Analyze Particles to measure the area of the image selected by the threshold.
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