Hello,
I am performing an assay where I pulldown bacterial cells with Ni-NTA magnetic beads to which my antibody is bound. The assay works (I get bacterial colonies when I plate the result, ~10^4 CFU/reaction), but the next step is to identify the bacterial species by analyzing their genomes. The problem is that the DNA is adsorbing onto the magnetic beads after I lyse the cells by heating the bead and cell mixture to 98 degrees for 10 minutes. If I cannot find a method to remove the DNA from the beads, can I have a recommendation for a protocol or kit that can purify DNA from a very small number of cells.
Thanks in advance
Do you have the possibility to separate the beads from the bacteria before lysis, e.g., by adding EDTA? Another option to try is changing the pH to ~2, in order to break the antibody-bacteria interaction.
Try using BDT (Big Dye Terminator) kit 3.1 from Thermo Fisher Scientific. They use ampure beads which only purifies the excess dimers or salts.
Eluting the bacteria from the beads before lysis was successful. Thank you everyone for your insight.
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