I am extracting RNA from Zebrafish tissue (Brain, Testes, Embryos etc) using standard Trizol total RNA extraction protocol. The RNA concentration and yeild are good. Checked on Qubit. RNA quality is very good too (eRIN >8). Checked on Agilent TapeStation.
On checking the RNA using a Nanodrop, it was found to have peaks indicating Trizol/Phenol contamination. How will this affect downstream steps for RNA-Seq library prep? The sequencing platform does not even do a Nanodrop check.
If anyone has similar experience or potential solutions, please let me know. Thanks.