Roy Mathew Francis

2 Questions 9 Answers 0 Followers

Questions related from Roy Mathew Francis

How do I prepare RNA-Seq libraries with Trizol contaminated RNA?

I am extracting RNA from Zebrafish tissue (Brain, Testes, Embryos etc) using standard Trizol total RNA extraction protocol. The RNA concentration and yeild are good. Checked on Qubit. RNA quality...

12 December 2015 3,537 5 View

Does nucleic acid staining affect sequencing?

Nucleic acids (DNA and RNA)/nucleus often needs to be stained to identify cell types and cellular location for use in confocal imaging, LCM (laser capture microdissection), flow cytometry etc....

04 April 2014 1,226 5 View