How do I prepare 0.04mM ATP solution from a 10mM stock solution?

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Are there any Bionic prostheses available like the ones in the images?

Greetings. I would like to ask if any working group has any bionic prostheses that I could obtain, I am attaching some examples in images. Thank you

16 April 2024 3,134 0 View

Problem with Mirdeep2 and Randfold output?

Hello team, I am using Mirdeep2 to identify miRNA in my organism (none model organism) which has no previously identified micro_RNAs. My organism is genetically close to Drosophila melanogaster....

14 April 2024 9,090 0 View

Where can I obtain a prototype similar to the Images?

I would like to obtain a prototype of a continuous robot similar to the one presented in the following paper: https://doi.org/10.3390/act3030226? I would like to obtain a prototype of a Bionic...

05 December 2023 8,203 0 View

Do the most recent advances in environmental chemistry have the potential to reduce greenhouse gas emissions?

One example of a recent method for carbon capture and storage is chemical reaction carbon capture and storage (CCUS). In this method, carbon dioxide is captured from flue gases and then stored in...

07 September 2023 6,347 4 View

What is the ultrasound intensity of the HC-SR04 sensor?

Nowadays, multiple areas of engineering use ultrasonic sensors, mainly due to their high precision within short distances and their robustness to electromagnetic interference. The well-known HC...

01 May 2023 3,166 2 View

Does this figure clearly explain the cycle of action research for health systems strengthening? If not, what advice would you give?

This figure has been adapted from Grodos et al. (2000) for application to the action research cycle for health systems strengthening that we wish to conduct. We need expert advice on this. The...

27 February 2023 8,133 2 View

Do I notify on the form of exogenous intoxication due to suicide attempt or on the self-inflicted injury form?

If I assist a patient with a case of self-poisoning in Brazil, do I need to report it on the exogenous intoxication form and on the self-harm form at the same time? Or maybe I should only report...

18 February 2023 8,694 1 View

CFD (ANSYS Fluent) Compressible Steady State Flow Through a Capillary into an Ion Funnel. Wondering why my parameters are causing the code to diverge?

I've worked on CFD for a capillary to diverging electro spraying from ambient nozzle spraying to a ion funnel under vacuum. Since I am hindered by computational power, I avoid making my flow go...

31 July 2022 7,327 0 View

ELISA target quantification accuracy?

Are ELISAs considered "semi-quantitative" due to the nature of antibodies being able to bind more than one antigen at a time? Since it is not a true 1:1 ratio, is it rational to assume that the...

04 March 2022 7,481 4 View

Could you give me some good sources about the principles of Metabolic Engineering?

I'm finding it very interesting but I don´t know how to continue with the topic. I have good fondation in genetic engineering.

03 August 2021 296 3 View

Methyl jasmonate preparation ?

How do I calculate the stock solution to apply 100 uM MeJA for 1000 ml of plant nutrient medium ?

01 August 2024 723 0 View

How do you get pure primary macrophage cell culture?

I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...

23 July 2024 4,074 2 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

15 July 2024 5,898 0 View

How to make 100ppm 1Liter methylene blue stock solution? How much weigh the dye require?

methylene blue stock solution

19 June 2024 10,030 3 View

What are impact of stagflation on emerging markets performance?

The emerging markets have suffered from a state of stagflation. I hope you know your expectations according to your experiences regarding the implications for the stock markets,,,, risks and...

19 June 2024 4,410 3 View

Why is iPSC in bad condition?

I saw the iPSC about a week after holding the stock (12 well and 3 stocks) and there were very few cells attached, and some cells were differentiated. I wonder what the problem is. The plate is...

12 June 2024 6,087 0 View

How to Dilute Stock solution of ferrous sulphate in frap assay?

I want to do Frap assay with ferrous sulphate heptahydrate (FeSO4.7H2O) as the standard. I have calculate the molarity which is 1 M or 1000 microMolar as the stock solution. How do I determine the...

11 June 2024 3,760 2 View

I want to ask if I suppose to add 200mg of antibiotic in 100ml of water so what is the concentration of this stock solution in microgram?

Hello, I recently started working with antibiotics and I do not have that much experience of making dilutions accurately. I want to ask if I suppose to add 200mg of antibiotic in 100ml of water...

08 June 2024 2,285 6 View

How can we conduct tests using p-nitrophenyl substrates?

How to establish the calibration curve of PNP in relation to concentrations in a microplate and for measuring the activity of lipase, what do we use? Substrate stock: 20 mM p-NP palmitate in...

19 May 2024 1,286 0 View

How to dilute my DNA Template( 1 Micromolar stock solution ) into1 × 10-8 nM to 1 × 10-15 nM ?

How can i dilute my master stock of 1 micromolar DNA template to different concentration by serial dilution

17 May 2024 3,641 2 View