Following Esposito et al. (2020), I am trying to understand and replicate the methodologies that have been used. However, I am stuck at the final two steps. The specifications are attached. Any help is appreciated.
I am using a vector that has repeat sequences, and using Mach1 competent cells. I heard that if the cells are not stable cells, then we shouldn't grow them in 37 to avoid recombination to happen...
26 June 2024 3,735 3 View
Dear Members of ResearchGate, I hope you are doing well. I have been retrofitting using the ASCE 41 2017 standard. In it, I have two basic doubts. 1. The target displacement as per clause...
13 June 2024 2,773 0 View
Hello Everyone, I hope you are doing well. What is the best book on seismic retrofitting reinforced concrete structures, preferably for Indian and US conditions (Recommendations please)? Thank...
30 May 2024 9,200 9 View
I am trying to understand how the expression of paralogues changed over time. I have real-time PCR data and gene tree. I want to reconstruct the estimated gene expression level at each node. I...
14 March 2024 8,131 0 View
Please can anyone suggest me how to write a book chapter in Elsevier?
21 February 2024 3,672 2 View
Hi Everyone, I want to know some of the best tools or platforms for planning research works (PhD or/and Master's research). Till now I have been using MS Excel to schedule my work, but it is not...
15 January 2024 6,985 3 View
We are purifying the protein from recombinant DNA
05 January 2024 1,086 2 View
Can someone please help me by providing the values of electronic properties (bandgap,electron and hole density of states, dielectric permittivity and electron and hole mobilities) of Niobium...
02 January 2024 3,962 1 View
Hi everyone. I wish you all a Merry Christmas and a Happy New Year ahead! I hope you are doing well. I have a question. In Opensees, why reinforced concrete beam-column joint is assumed as a...
25 December 2023 3,540 3 View
A micellar solution of protein (in PBS, pH 7.4) was loaded into the sample cell of the calorimeter. The ligand was prepared in 10% DMSO and was loaded in the injector-stirrer syringe. I have also...
22 August 2023 3,630 16 View
I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...
08 August 2024 719 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hello everyone
01 August 2024 6,431 4 View
I went for Plackett Burman Design for selecting the optimization factors of growth conditions for pesticide degradation. I started with 7 parameters. But, after the experiments and analysis, only...
29 July 2024 5,015 1 View
Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?
27 July 2024 648 3 View
My thesis includes variables of inflation rate (monthly), interest rate (weekly), exchange rate (daily), and crude oil production (monthly), as well as imports and exports (annually). The period...
27 July 2024 7,384 5 View
I am trying to put 3 separate plasmids into Staphylococcus aureus and I am looking for those that are theta replicating and compatible. I have obtained pSK9065 (from pSK1) from Addgene, but the...
23 July 2024 6,968 0 View
Kao's panel cointegration tests , is there anyone willing to explain me the eviews-9 output for the Kao's panel cointegration tests?
23 July 2024 5,051 4 View
I am looking for journals that admit the publication of results from applying the IUCN Standard for designing and monitoring Nature-based solutions. Many thanks for considering my request.
23 July 2024 6,444 2 View