I am running a titration for IHC assay. Primary antibody is Calreticulin polyclonal antibody (PA3-900), secondary antibody is Alexa fluor 488 Goat anti-rabbit IgG (A11008).
The goal is to see the expression of calreticulin in treated and untreated tumor cells.
Recommended dilutions were as following
1Ab (1:50), (1:100) & (1:200)
2Ab (1:200), (1:100) & (1:200)
Questions?
Are these dilutions good enough to start with?
Would the untraded and treated sample be enough to do a comparison ?
Mayank Choubey
If you have sufficient Ab then go for IHC or IF Otherwise go for western. You may also try dilution 1:25 or 1:30.
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