Aman Az

2 Questions 1 Answers 0 Followers

Questions related from Aman Az

How do I perform a proper antibody titration for IHC assay?

I am running a titration for IHC assay. Primary antibody is Calreticulin polyclonal antibody (PA3-900), secondary antibody is Alexa fluor 488 Goat anti-rabbit IgG (A11008). The goal is to see the...

11 April 2017 6,973 3 View

What is the best antibody dilution buffer recipe for fluorescence IHC

I am running a fluorescence IHC to detect calreticulin in paraffin embedded tissue. Primary antibody is polyclonal calreticulin and secondary antibody is Alexa Flour. Using 0.05% Tween 20, 3%...

01 January 1970 4,233 3 View