How do I maximize transformation efficiency in yeast (S. cerevisiae) gap repair cloning?

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Christian Q. Scheckhuber

Hello Christopher,

BY cells can be difficult to work with. In many ways they don't behave as good yeast cells should. By the way, I found an article which gives some suggestions on how to improve gap repair in yeast. Maybe it is of use to you?

With best regards, Christian

Article Improved gap repair cloning in yeast: Treatment of the gappe...

Christopher Bystroff

That is useful! thank you

Saad Alaraji

Hello,

I think that you can't get very many transformants.

Good Luck