Christopher Bystroff

3 Questions 16 Answers 0 Followers

Questions related from Christopher Bystroff

How do I maximize transformation efficiency in yeast (S. cerevisiae) gap repair cloning?

Standard protocols for gap repair cloning call for 30b upstream and downstream homology regions. We use 50b. We want to insert a library into a vector, but get very few transformants. (using pYES...

13 July 2017 4,921 3 View

What is the best approach to overpopulation?

Too many humans! It is the underlying cause of all things anthropogenic. Ehrlich and Holdren's equation sums it up: I=PAT. Ecological impact (I) is proportional to the human population (P) times...

01 January 1970 5,259 2 View

Could anti-glycan autoantibodies be the cause of COVID-19-related multisystem inflammatory syndrome in children (MISC)?

SARS-COv-2 spike glycoprotein is covered with glycans. Anti-glycan auto-antibodies are known to exist in humans and are implicated in autoimmune diseases (Bovin et al, BBA , 2012, p.1373) but are...

01 January 1970 5,683 0 View