If I want to knock out or knock down an RNA binding protein, are there any specific considerations to keep in mind? How can I make sure that the wildtype reconstitution is able to bind RNA and rescue phenotype?
The title of the question does not match your request. Justify your question/request more precisely.
Your Bro
23 April 2024 251 1 View
Hi, I need the MATLAB code for the Centroid and APIT localization algorithms (wsn) to verify the results I obtained with the DVHOP algorithm. Thank you in advance. Best regards.
05 February 2024 6,119 4 View
Hello everyone, I am a beginner in computational studies. Little familiar with Material Studio. I modified the ZIF-8 structure with an ethyl group. After modification, when the geometry was...
29 January 2024 1,145 0 View
The estimated sample size was 280 according to the population (registered pharmacist). Since I am going to use two diffrent prescriptions to evaluate their practice, will the sample be 280×2 or...
29 December 2022 5,055 6 View
I did an RNA-IP out of cells and my RNA yield for the inputs was great but the yield was poor for the IP samples. Is there a way to increase the yield of RNA? (other than increasing the starting...
27 November 2022 2,847 8 View
I'm trying to detect the local Nu number at different planes. My issue is that when I open the variable option in chart, I can't get the "surface Nusselt number" to show up under the variables...
31 January 2022 7,327 2 View
Hello, I have a problem with the detection of the small RNA ladder on the bioAnalyzer for the quantification of small RNAs. I contacted Agilent, they sent me another ladder. I had the same...
05 January 2022 6,215 0 View
Hello Dear rima I want a software model to simulate photodetectors by the simulation software Silvaco means the most important models in it, especially if the photodiode is from a Schottky...
24 October 2021 8,555 3 View
Dear all, I have to realize GC-MS and HPLC for my bacterial extract (the solvent used was ethyl acetate). I would like to know how could I choose the experimental conditions for that (injection...
18 April 2021 1,163 6 View
I am using visual perspective but the study needs the verbal aspect as well to be analysed for more comprehensive research. Thank you in advance
18 March 2021 1,130 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View