Why do we use HHL as a substrate in studying ACE enzyme inhibition activity assay?

Need to know from the community why we use Hippury-L-histidyl-L-Leucine as a substrate in ACE inhibitory activity

20 September 2020 1,040 5 View

Just noticed with some papers that when we use Box-Behnken design for optimization using RSm, there are three identical runs. Can anyone explain why?

I ran a experiment in design expert and obtained three identical runs which seems weird. Checked few papers and they have three identical runs too and are published. Whats the reason behind this?

14 September 2020 6,091 8 View

Can I store isolated RNA on -20 degree centigrade?

I have isolated RNA from animal tissue. Can I store it on -20 degree centigrade?

04 December 2019 1,888 5 View

What are Open Source AI tools that could be used for Automatic Extraction of Exposure data from high resolution satellite imegeries (<0.5 m) ?

Open source AI tools could be great help.

05 February 2019 7,172 3 View

If Mars was livable, would you accept a one-way ticket there?

It would take guts, a sense of adventure, and maybe a reason to run away, all great to explore in conversation.

22 January 2019 9,649 7 View

What are the different convergence criteria to check whether the solution is truly converged or not?

Is it sufficient to judge convergence only by examining residual levels while modeling fluid flow and heat transfer problems? If not, what are the different metrics used for judging convergence?

02 September 2018 3,652 5 View

What are the core infrastructure facilities required in a smart city?

31 August 2018 1,857 5 View

How to convert topology and trajectory files from gromacs to amber?

I want to use cpptraj for some analysis but my topology and trajectory file is in .top and .xtc or .trr format. Please give me resoponse for how to run parmed command in a proper way to convert...

08 August 2018 8,700 5 View

Which type of sequencing is best suited for 300bp PCR product?

Hello every one, Can you please suggest me which method should I use for sequencing a 300bp PCR products either Illumina or Oxford Nanopore. I have around 100 PCR products which are about 300bp long.

31 October 2017 6,091 8 View

Is it possible to make Titanium oxide film from titanium trichloride?

I am working on rGO-doped TiO2 thin film for IR reflection. Can I make TiO2 film by Titanium trichloride??

26 October 2017 3,703 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View