The question is not very specific. In general if you are looking for an enzyme from a bacterial culture you can separate the cells by centrifugation, and  using your enzymatic assay you can check the presence of enzyme of your interst in the extracellular medium.

secondly you can crush the cells by high pressure homogenization and concentrate the protein content and check the presence of your enzyme by activity assay.

 If you want a standardized protocol you can elaborate your question more and particularly for which enzyme you are looking for?

For each enzyme you can use different activity assay separately. 

Hope it willl help you.

A Process for large-scale isolation of beta-galactosidase from E. coli in an aqueous two-phase system.

Veide A1, Smeds AL, Enfors SO.

kindly read above journal

Thank you for your reply !

I Want to use beta glucosidase enzyme for hydrolyzing my biomass.

I want to isolate crude enzyme from microbes or any other sources.As using pure commercial enzyme is bit coastly practically.

Kindly help me out with this

okay 

the isolation method  of crude protein content from microbial cells are always similar.

For better understanding of enzyme isolation read these papers carefully. Set up a reaction system to check the activity of beta glucosidse as given in these articles.

Your first step is to purify the enzyme with high activity. Please follow these papers

http://www.sciencedirect.com/science/article/pii/S0964830515300895

http://www.sciencedirect.com/science/article/pii/S1359511315301069

http://www.sciencedirect.com/science/article/pii/S0141022914000258

Hope it will help you.

BEST LUCK WITH YOUR RESEARCH

okay 

the isolation method  of crude protein content from microbial cells are always similar.

For better understanding of enzyme isolation read these papers carefully. Set up a reaction system to check the activity of beta glucosidse as given in these articles.

Your first step is to purify the enzyme with high activity. Please follow these papers

http://www.sciencedirect.com/science/article/pii/S0964830515300895

http://www.sciencedirect.com/science/article/pii/S1359511315301069

http://www.sciencedirect.com/science/article/pii/S0141022914000258

Hope it will help you.

BEST LUCK WITH YOUR RESEARCH

The first point to note is the types of enzymes of interest. Are the enzymes In plants, animal organs, fungi, yeast culture or what? Are the enzymes found within the cells or in the growing medium. Enzymes produced in the growing medium can easily be obtained by centrifugation. But if the enzyme is found in the cells of organisms then crushing of the cells either by sonification process will do, and followed by centrifugation. You also need to develop a protocol for identifying and quantifying the enzyme of interest.