How to count the number of figure when reusing figure??

Dear all I'm preparing a review paper and confused about terms for reusing figure in other papers... Can you please help me for 2 question..?? (1) As i know, the cost of reusing figure are...

28 December 2020 1,515 3 View

[ELI5] FRET assay, Protease assay, Michelis Menten. How would you convert the fluorescence signal to the velocity (concentrion/time)?

Hello i am fairly new to the field of physical chemistry and only have a vague memory of it from undergrad classes. my FRET assay is set up like a regular FRET assay, so the enzymetic activity of...

18 June 2020 9,730 4 View

How to calculate blood pressure variability of specific bandwidth?

We are currently trying to perform spectral analysis on blood pressure with matlab. The device we used(Caretaker, Biopac) provides time position and amplitude of the peak according to the PDA...

18 November 2019 448 3 View

Western blot doesn't work well, what's a problem?

Recently, I got a problem on western blot. Smaples are not apart as molecular size, and even marker is not apart from about 70kb to 130kb. I did make a new gel buffer twice, changing tris and 10%...

04 July 2018 1,410 6 View

How can I directly transfer graphene on SiO2/Si?

Hi. I'm proceeding the graphene transfer on SiO2/Si directly, not using Al2O3 to increase the adhesion. The method that I used is as follow; 1) Spin coat the PMMA on the graphene on Cu foil...

01 December 2017 8,579 8 View

Fusion protein western blot?

I made recombinant fusion protein by DNA cloning. C-term of my target protein has some tag for detection and purification. For example, Targert- His6-GST When I did western blot analysis using...

19 November 2017 143 5 View

Protein transfer to membrane after coommasie staining

I already check protein expression level on SDS-PAGE gel. For checking I already used CBB stainning. But I'm not sure it was my target protein or not. So on this gel, I want to do transfer to...

18 July 2017 5,077 2 View

Can anyone help with LC3 expression and Rapamycin /Bafilomycin treatment?

08 January 2015 9,529 6 View

In this system, is the methanol a poor solvent for Au nanoparticles? (DT-Au)

I am doing a synthesis of gold nanoparticles preparation method by Brust et al. , to study its size variations by temperature control, and I have a question in one of the synthesis steps of the...

06 March 2014 957 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View