How to count the number of figure when reusing figure??

Dear all I'm preparing a review paper and confused about terms for reusing figure in other papers... Can you please help me for 2 question..?? (1) As i know, the cost of reusing figure are...

28 December 2020 1,515 3 View

[ELI5] FRET assay, Protease assay, Michelis Menten. How would you convert the fluorescence signal to the velocity (concentrion/time)?

Hello i am fairly new to the field of physical chemistry and only have a vague memory of it from undergrad classes. my FRET assay is set up like a regular FRET assay, so the enzymetic activity of...

18 June 2020 9,730 4 View

How to calculate blood pressure variability of specific bandwidth?

We are currently trying to perform spectral analysis on blood pressure with matlab. The device we used(Caretaker, Biopac) provides time position and amplitude of the peak according to the PDA...

18 November 2019 448 3 View

How do I import a forder that contains a txt file in EEGLAB ?

file > import data > using the FILE-IO interface. When importing the folder, an unsupported header format "unknowm" error will occur. What is the solution?

11 April 2019 3,895 2 View

Western blot doesn't work well, what's a problem?

Recently, I got a problem on western blot. Smaples are not apart as molecular size, and even marker is not apart from about 70kb to 130kb. I did make a new gel buffer twice, changing tris and 10%...

04 July 2018 1,410 6 View

How can I directly transfer graphene on SiO2/Si?

Hi. I'm proceeding the graphene transfer on SiO2/Si directly, not using Al2O3 to increase the adhesion. The method that I used is as follow; 1) Spin coat the PMMA on the graphene on Cu foil...

01 December 2017 8,579 8 View

Fusion protein western blot?

I made recombinant fusion protein by DNA cloning. C-term of my target protein has some tag for detection and purification. For example, Targert- His6-GST When I did western blot analysis using...

19 November 2017 143 5 View

Protein transfer to membrane after coommasie staining

I already check protein expression level on SDS-PAGE gel. For checking I already used CBB stainning. But I'm not sure it was my target protein or not. So on this gel, I want to do transfer to...

18 July 2017 5,077 2 View

Can anyone help with LC3 expression and Rapamycin /Bafilomycin treatment?

08 January 2015 9,529 6 View

In this system, is the methanol a poor solvent for Au nanoparticles? (DT-Au)

I am doing a synthesis of gold nanoparticles preparation method by Brust et al. , to study its size variations by temperature control, and I have a question in one of the synthesis steps of the...

06 March 2014 957 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View