To check the difference in hydrophobic expose level, we performed a fluorescence assay using ANS. However, after the measurement, the measured value came out in a unit called RFU, so I would like to convert the unit to fluorescence intensity (A.U).
Fluorescence intensity is measured in arbitrary units, unlike absorbance. RFU stands for relative fluorescence units. That is the unit of fluorescence intensity. You can compare the intensities of samples to each other as long as the measurements are made under identical conditions on the same instrument, preferably at the same time.
The reason fluorescence intensity is measured in arbitrary units is that the amount of light detected depends on numerous factors, including several instrumental ones and sample geometry.
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