I plan to use the kit from life technologies using CRISPR nuclease vector kit.
you might use the online tool created by Zhang lab at http://crispr.mit.edu/
which regression analysis will be applied if there are 7 nominal dependent variables(YES/NO categories) and 1 ordinal independent variable(3 categories: Low, normal, high)?
23 April 2024 9,971 3 View
While making forest plot Revman I label the plot as Left control and right experiment. I see that the data supports the control as the diamond is on the left. But when I change labels the...
25 February 2024 3,144 2 View
How grain size effects the antibacterial properties?
30 January 2024 6,225 1 View
Techniques Tools required Step by step Procedure
21 January 2024 8,960 0 View
In CSt how do we calculate Power handling capacity of h plane horn antenna
12 December 2023 1,713 0 View
I've tried the liquid exfoliation method but I'm not getting the results. If anyone else has tried through this method can anyone share protocols so that I can check where I'm making mistakes in...
10 September 2023 3,753 0 View
Hi everyone, I have problem while saving the computed points after adding the break lines in 2d unsteady flow. here i have attached the image of the problem I'm facing.
07 September 2023 2,235 0 View
I am conducting CV analysis of a rGO-based electrode using CHI 650E workstation with platinum foil as counter electrode, an Ag/AgCl reference electrode, and 1M H2So4 electrolyte. How can I decide...
07 September 2023 5,403 4 View
I got negative potential window in the range of -0.6 to 0.3 for 3 electrode system ,so can I use negative voltage window for -0.6 to 0.4 for 2 electrode supercapacitor system.
03 August 2023 1,765 0 View
while I am performing EIS in Autolab (NOVA 2.1.6) Metrohm,there are three types of options are available .what is difference between them .
27 July 2023 4,499 0 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View