How do I fixate cells without violet staining in a wound healing assay?

29 December 2021 1 4K Report

Working on some cell culture & different lines, does anyone have a specific protocol for fixing cells with formalin & using Triton X-100 to fix cells?

Jayant Dewangan

Fixing cells with 100% Methanol followed by staining with 0.1% Crystal Violet works good.

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