i used different restriction enzymes to cut plasmid DNA and ran this with a transcription mixture on the gel electrophoresis
I want to synthesize about 2.5kb cDNA and I need a suitable kit.
20 November 2023 5,196 4 View
I am currently carrying out a study on Africans perspective on conservation and I am having a hard time getting papers to refer to as it seems not many researches have been done in that area. I...
28 January 2021 3,918 2 View
The Census Method is also called as a Complete Enumeration Survey Method wherein each and every item in the universe is selected for the data collection, or whenever the entire population is...
21 May 2020 4,665 16 View
Genomics of diabetes mellitus......can this in anyway improve nursing care for diabetic patient?
17 November 2019 3,708 2 View
I am working on the study of geometry and combustion analysis in a SI engine. I will like to use software that will carter for chemical kinetics and thermo properties of the species of the...
15 January 2018 245 3 View
how can emotional intelligence and job affect predict job satisfaction among nurses
26 January 2016 1,766 3 View
Please how can I analyse inhibitor film formed on the surface of mild steel?
18 October 2015 5,717 2 View
I have some samples whose structures I want to elucidate. I therfore need relevant information such as postal addresses, email addresses, phone numbers, websites etc of laboratories, institutions,...
03 June 2015 7,024 7 View
Due to economic and ethical reasons, the use of in vitro tests in ethnopharmacological studies has become very popular and widespread (despite the fact that in vivo models give a more accurate...
17 November 2014 8,770 2 View
Anticancer testing of chemical agents. What that are the possible organic solvents that will be innocuous to the cancer cells.
06 May 2014 4,776 2 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...
30 July 2024 1,412 8 View
through genetic modification or metabolic engineering? Is it helpful, good for health ?
22 July 2024 7,811 4 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
What happens in snapTotal- seq? (Single-cell Total-RNA Profiling Unveils Regulatory Hubs of Transcription Factors https://www.nature.com/articles/s41467-024-50291-3) In this protocol, several RT...
18 July 2024 10,010 0 View
I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...
17 July 2024 6,213 4 View