Please, what kit can synthesize long cDNA; citation would be appreciated?

Hello Christianah, Have you tried Induro® Reverse Transcriptase (#M0681S) with NEB . Its relatively new product and also I have not used it personally. But recently while optimizing a protocol for long cDNA synthesis I had really great recommendation for using this enzyme over others. (Link below)

https://www.neb.com/en-us/products/m0681-induro-reverse-transcriptase#Product%20Information

Best luck...!!!

Aarya Chitransh

There are several kits available for synthesizing long cDNA, and the choice of kit often depends on the specific requirements of your experiment. One popular method for synthesizing long cDNA is the use of a reverse transcription kit with a combination of random primers and oligo(dT) primers. Below are a few commercially available kits known for their ability to generate long cDNA fragments:

SuperScript IV Reverse Transcriptase Kit:Manufacturer: Thermo Fisher Scientific Description: This kit is known for its high processivity and can be used for efficient cDNA synthesis from RNA templates, including long transcripts. Citation: Cheng C, et al. (2019). A Comprehensive Characterization of the Function of the 3' Exonuclease Suppression in the Viral RNA Polymerase from the Coronavirus. Journal of Biological Chemistry, 294(43), 16284–16295.

SMARTer Universal Low Input RNA Kit for Sequencing v4:Manufacturer: Takara Bio Description: This kit utilizes SMART (Switching Mechanism at 5' End of RNA Template) technology to generate full-length cDNA from low-input RNA samples. Citation: Tariq MA, et al. (2011). RNA-seq analysis of coronary artery gene expression reveals a potential role for the Tie2 pathway in vein graft disease. Physiological Genomics, 43(12), 799–810.

QuantiTect Reverse Transcription Kit:Manufacturer: QIAGEN Description: This kit is designed for sensitive and quantitative cDNA synthesis. It includes a unique genomic DNA elimination step and is suitable for long RNA templates. Citation: Wong ML, et al. (2015). Whole-blood gene expression in adolescent chronic fatigue syndrome: an exploratory cross-sectional study suggesting altered B cell differentiation and survival. Journal of Translational Medicine, 13, 299.

RevertAid First Strand cDNA Synthesis Kit:Manufacturer: Thermo Fisher Scientific Description: This kit includes RevertAid Reverse Transcriptase, which is known for its high sensitivity in cDNA synthesis. It is suitable for long cDNA synthesis from RNA templates. Citation: Cui Y, et al. (2019). Hes1 Promotes the IL-22-Mediated Antimicrobial Response by Enhancing STAT3-Dependent CEBPε Production in a T Helper 17 Cell Line. Journal of Immunology, 203(8), 2088–2101.

Always check the manufacturer's protocol and recommendations for each kit to ensure that it meets your specific requirements for synthesizing long cDNA. Additionally, consider the specific RNA templates you are working with and any unique features of your experimental design.

Amy Klocko

I've used a kit from Invitrogen before, it works well and the kit parameters state it can be used for products up to 11 KB in size. Of course you'll need good quality RNA as the input.

Verso cDNA Synthesis Kit (thermofisher.com)

Christianah Odesanmi

Thanks @ all for all your recommendations

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