Dearest colleagues,
I am writing a proposal for a project concerning IBD research and substitution according to the 3R principle. Therefore I would like to know the actual numbers of animals (mouse, rat, etc.) used for TNBS, DNBS and DSS induded colitis in the years 2017-2019.
I tried the official announcments (e.g. European statistic report on the use of animals), but I only find general information ( e.g. # animals in gastrointestinal research).
Do you have a suggestion how to easily address that matter in short time?
I started now by looking at each publication of each year and manually collecting the numbers, however this is really time consuming. Maybe there is a automated approach.
I am looking forward to any suggestion, thank you very much in advance.
This is a difficult question, because I think depends on a lot of question like: how many groups you use in your study? How many days these protocols have? In my opinion, you should try to do a pilot if your laboratory don't have experience and said that is according to 3D principle in your project. This link speaks more about simple size http://www.3rs-reduction.co.uk/html/6__power_and_sample_size.html . This depends on of resource equation.
I hope its helps you.
@Maria Jara Montibeller
Thank you for your suggestions.
I found a good website for German animal testing numbers
https://www.animaltestinfo.de/
For every animal project you find a ntp, which gives you the planned animals for this study. You can also specify your search with keywords.
Hi,
I think now a days you need to define the sample number of your experiment. whether you submitting a grant or article, you need to follow it at several places. The best way is to calculate by itself by using various software's which you can find in the recent literature of papers submitted to Nature, Cell publications by going to star methods.
Another method is to go through various studies which related to your experiment design and deduce the common number of animals they have used.
you need to take into consideration various parameters while defining your sample size, like the type of your study, quality of your animal facility, mortality of species etc.
I hope it is somewhat useful to you!
Hamid