My protein of interest is a transmembrane plant kinase with a putative malectin-like domain. I would like to find out which carbohydrate(s) it binds, and/or which protein(s) it phosphorylates or is associated with in a less transient complex.
With it being a kinase and transmembrane protein, findings a suitable method for identifying (transient) interaction partners has proven difficult!
So I'm happy to receive any suggestion!
Almudena Pino Angeles
Hello,
I understand you want to do it computationally, right? If so, the attached publication may give you an idea.
Article Systematic computational prediction of protein interaction networks
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Sébastjen Schoenaers
Hi Almudena,
I'm not looking for computational approaches but I'm happy to have a look at it anyway. Thanks for the suggestion.
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