Sébastjen Schoenaers

3 Questions 18 Answers 0 Followers

Questions related from Sébastjen Schoenaers

How do I find interaction partners for a transmembrane malectin-like kinase?

My protein of interest is a transmembrane plant kinase with a putative malectin-like domain. I would like to find out which carbohydrate(s) it binds, and/or which protein(s) it phosphorylates or...

17 September 2015 9,240 2 View

Is it possible to rescue the kanamycin resistance in Arabidopsis SALK lines?

The seeds I got from NASC have no kanamycin resistance despite having the NPTII gene/T-DNA insertion. I tried growing seedlings on 5'-aza-2'-deoxycytidine to reset any methylation related...

23 January 2015 6,212 5 View

Can one do gateway constructs with genomic DNA instead of cDNA?

I'm creating overexpression, N- and C-terminal protein GFP fusions, PROM.GFP, and PROM.GUS lines for my Arabidopsis gene of interest. I've been using classic gateway cloning. The question is: Can...

19 November 2014 5,388 9 View