Hello, use a culture filtrate ratio of 1:1(v/v) with ethyl acetate and shaken, then take ethyl acetate layer and remove the solvent by vacuum evaporation at 40"C and dissolve obtained residue in methanol for purification and filtration.
Karen A. Darbinyan
Thank you for the kind answers.
Based on your advice,
Thank your so much!!
Reference these papers and also it is best for ethyal acetate extraction most of the papers used.
https:// doi.org/10.1371/journal.pone.0200500
, Article Extraction and purification of an antimicrobial bioactive el...
Sreenivasa NayakaThank you so much to help me solve the problem.
How do you mean "extraction of antimicrobial from Streptomyces"? First demonstrate antibiosis with the culture filtrate of the bacterium (filamentous). separate cells from broth and obtain cell-free extract (sterile broth). Use the "Eye of the organic Chemist - the thin layer chromatography. Confirm bio-activity of fractions that migrate. Note the solvent system that enabled it (them) and employ same in solvent extraction. It's a lovely process but cumbersome. Remember there are different chemical forms of bio-active material. You've got some work to you
Thanks Dr. Gaber El-Saber Batiha
I think this attached paper contains detailed information about the Isolation and purification of the antibacterial compound from Streptomyces levis collected from a soil sample.
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