First remove mucilage from plant tissue by using organic solvent then follow the standard method for proteins
For quantitative determination of proteins, Lowry's or Bradford's method is widely used.
first remove mucilage from plat material using organic solvent.Then extract 2g of plant material with 5ml distilled water.Now to 2ml of this extract add 5ml 10% cold TCA to precipitate protein.Take the precipitate and digest it with about 2ml conc.sulphuric acid at low temp.(25-30*C)till it turns apple green.Now make the volume 5ml by adding distilled water.From protein can be estimated following standard method of Lowry et al(1951)or any other method
Hi you can precipitate proteins using 5% TCA and follow the standard procedures for proteins.
Barraclough, D., Obenland, D., Laing, W., & Carroll, T. (2004). A general method for two-dimensional protein electrophoresis of fruit samples. Postharvest Biology and Technology, 32(2), 175-181.
The extraction method here is very effective for tricky tissues.
Do u interested in specific proteins or total seed proteins?
The best source for protein profile studies in mucilaginous plants is seeds.
How do u differentiate portuluca oleraceae from portulaca qudrifida .
Leaf apex of Portulaca oleracea is obtuse and for P. quadrifida it is acute. Color of petals in P. oleracea is yellow and for P. quadrifida it is pink. Moreover, capsule structure can also differentiate these species. I think these characters are sufficient to state difference between these plants. For more information you can search on http://www.tropicos.org/.
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany
- Plant Biology